Study Results of a New Centrifugal Pump for Medical Perfusion Complex to Restore and Maintain the Viability of the Isolated Donor Liver

摘要

Objectives: The article deals with experimental studies of original centrifugal pump developed in the Central Research Institute of Robotics and Technical Cybernetics (Saint-Petersburg, Russia) and designed for the use in apparatus for machine perfusion of the donor liver. Methods: In 4 series of experiments the traumatic effect of the pump on blood cells and changes in certain parameters during the perfusion with donor blood were studied. Blood samples were taken before the perfusion and at 60, 120, 240 minutes of the perfusion. Blood samples were analyzed using spectrophotometer and hematologic analyzer. A study of pressure-flow characteristics of the centrifugal pump incorporated into the developed apparatus for machine perfusion with arterial and venous lines was also performed. Findings: After 4 hours of donor blood circulation in the closed circuit by means of the studied pump there were no significant changes in blood parameters except for increase in free hemoglobin concentration which was comparable to what happens during the perfusion with a roller pump. The centrifugal pump provides pressure-flow characteristics required for perfusion of donor liver both in arterial and venous lines. In the operating area of hydrodynamic characteristics of the pump incorporated into the apparatus for machine perfusion of donor liver the fluid pressure in the arterial and venous lines reaches 2.7 and 2.3 m, respectively. Improvements: In experimental study it was shown that original centrifugal pump is safe and effective in terms of blood perfusion and it looks promising for the use in the apparatus for machine perfusion of the donor liver.