Reclamation of Ampicillin Sensitivity for the Genetic Manipulation of Legionella pneumophila

Molly C. Sutherland; Joseph P. Vogel

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Reclamation of Ampicillin Sensitivity for the Genetic Manipulation of Legionella pneumophila

机译：氨苄青霉素敏感性的回收对嗜肺军团菌的遗传操纵。

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Research on Legionella pneumophila, the causative agent of Legionnaires' disease, has been hampered due to the lack of selectable markers for genetic manipulation. We report the construction of a mutant strain of L. pneumophila lacking loxA, a chromosomally encoded β-lactamase, that has enhanced sensitivity to ampicillin. Also described are a method for converting Legionella strains to ampicillin sensitivity and conditions for utilizing bla as a selectable marker.

机译：军团菌病的病原体肺炎军团菌的研究由于缺乏基因操作的选择性标记而受到阻碍。我们报道了缺乏 loxA （一种染色体编码的β-内酰胺酶）的对肺炎链球菌突变株的构建，该株对氨苄青霉素的敏感性增强。还描述了将军团菌菌株转化为氨苄青霉素敏感性的方法，以及利用 bla 作为选择标记的条件。 展开▼

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《Applied and Environmental Microbiology 》 |2012年第15期| 共3页

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Molly C. Sutherland; Joseph P. Vogel;
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中图分类植物学 ;

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1. Reclamation of Ampicillin Sensitivity for the Genetic Manipulation of Legionella pneumophila [J] . Molly C. Sutherland, Joseph P. Vogel Applied Microbiology . 2012 ,第15期

机译：氨苄青霉素敏感性的回收对嗜肺军团菌的遗传操纵。

2. Immunochromatic kits Xpect Legionella and BinaxNOW Legionella for detection of Legionella pneumophila urinary antigen have low sensitivities for the diagnosis of Legionnaires’ disease [J] . Christina Wiid Svarrer, Christian Lück, Pernille Landsbo Elverdal, Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland . 2012 ,第2期

机译：用于检测嗜肺军团菌尿液抗原的免疫染色试剂盒Xpect军团菌和BinaxNOW军团菌对诊断军团菌病的敏感性较低

3. Cross-reactivity and sensitivity of two Legionella urinary antigen kits, Biotest EIA and Binax NOW, to extracted antigens from various serogroups of L-pneumophila and other Legionella species [J] . Okada C, Kura F, Wada A, Microbiology and Immunology . 2002 ,第1期

机译：两种军团菌尿液抗原试剂盒Biotest EIA和Binax NOW的交叉反应性和敏感性对从L-肺炎衣原体和其他军团菌属各种血清群中提取的抗原具有交叉反应性和敏感性

4. Reliable recognition of Legionella spp. And Legionella pneumophila from watersamples by the new immunoassay Duopath Legionella [C] . J. H. HELBIG, P. C. LüCK, A. BUBERT, 2006 WQTC (Water Quality Technology Conference) . 2006

机译：军团菌属的可靠识别。新型免疫测定方法Duopath Legionella从水样中分离出肺炎军团菌和军团菌

5. Revealing Phylogenetic Relationships and CRISPR-Cas Functionality in Legionella pneumophila using Next Generation Sequencing [D] . Rao, Chitong 2017

机译：使用下一代测序揭示嗜肺军团菌的系统发生关系和CRISPR-Cas功能

6. Reclamation of Ampicillin Sensitivity for the Genetic Manipulation of Legionella pneumophila [O] . Molly C. Sutherland, Joseph P. Vogel 2012

机译：氨苄西林敏感性的回收对嗜肺军团菌的遗传操纵。

7. Reclamation of ampicillin sensitivity for the genetic manipulation of Legionella pneumophila [O] . Sutherland Molly C, Vogel Joseph P 2012

机译：回收氨苄西林敏感性对嗜肺军团菌的基因操作

8. Growth of 'Legionella pneumophila' in Continous Culture and Its Sensitivity to Inactivation by Chlorine Dioxide [R] . Berg, J. D. , Hoff, J. C. , Roberts, P. V. , 1984

机译：连续培养中'嗜肺军团菌'的生长及其对二氧化氯灭活的敏感性

1. 四种微藻对氨苄青霉素敏感性的研究 [J] . 岳伟萍 . 水产养殖 . 2005 ,第006期

2. 四种微藻对氨苄青霉素敏感性的研究 [J] . 岳伟萍 . 河北渔业 . 2005 ,第004期

3. 氨苄青霉素二氯甲烷回收工艺研究 [J] . 冯柏春 . 科技致富向导 . 2015 ,第002期

4. 氨苄青霉素二氯甲烷回收工艺研究 [J] . 王连君 ,刘春玲 . 黑龙江科技信息 . 2004 ,第004期

5. 血糖正常人群的胰岛素分泌及敏感性与2型糖尿病遗传背景遗传强度的相关性研究 [J] . 陆晓敏 ,闾倩 ,宋艳 . 中国实用内科杂志 . 2017 ,第005期

6. 从捷克猪分离的产气荚膜梭菌A型cpb2+对阿莫西林、氨苄青霉素(氨苄西林)和复方阿莫西林克拉维酸的体外敏感性 [C] . Masarikova Martina ,周远成 . 第22届国际猪兽医协会大会（IPVS2012） . 2012

7. 中国城市自然水环境中军团菌分布情况调查及嗜肺军团菌的遗传多态性研究 [A] . 郭景玉 . 2015

1. 一种体外套式环介导等温扩增法的检测嗜肺军团菌的试剂及检测嗜肺军团菌的方法 [P] . 中国专利： CN105483211B . 2018.11.06

2. 一种体外套式环介导等温扩增法的检测嗜肺军团菌的试剂及检测嗜肺军团菌的方法 [P] . 中国专利： CN105483211A . 2016-04-13

3. PRIMER FOR AMPLIFICATION OF TARGET BASE SEQUENCE OF LEGIONELLA BACTERIA, METHOD FOR AMPLIFYING TARGET BASE SEQUENCE OF LEGIONELLA BACTERIA, PRIMER FOR AMPLIFICATION OF TARGET BASE SEQUENCE OF LEGIONELLA PNEUMOPHILA AND METHOD FOR AMPLIFYING TARGET BASE SEQUENCE OF LEGIONELLA PNEUMOPHILA [P] . 外国专利： JP2005211027A . 2005-08-11

机译：用于扩增嗜血菌的靶基序列的引物，用于扩增嗜血菌的靶基序列的方法，用于扩增嗜血菌的肺炎双歧杆菌的靶基序列的引物和用于扩增的方法

4. PRODUCTION METHOD, AND FILTER COMPRISING NON-WOVEN FABRIC AND/OR FILTERING INJECTOR STRUCTURES OR SHEETS WHICH ARE OBTAINED USING SAID METHOD AND WHICH ARE INTENDED FOR THE FILTRATION AND ELIMINATION OF LEGIONELLA PNEUMOPHILA IN ANY INSTALLATION WITH A RISK OF LEGIONELLA PNEUMOPHILA PROLIFERATION [P] . 外国专利： KR101464206B1 . 2014-11-21

机译：的生产方法，以及过滤器，其中包含使用常规方法获得的非织造织物和/或过滤性注射剂结构或片材，并打算通过任何形式的囊突装填法消除和消除肾小球囊小核糖核酸

5. Production method, and filter comprising non-woven fabric and/or filtering injector structures or sheets which are obtained using said method and which are intended for the filtration and elimination of Legionella pneumophila in any installation with a risk of Legionella pneumophila proliferation [P] . 外国专利： AU2005227099B2 . 2010-04-22

机译：生产方法以及包括无纺布和/或过滤注射器结构或片材的过滤器，所述无纺布和/或过滤器注射器结构或片材是使用所述方法获得的，并且意在用于过滤和消除嗜肺军团菌的任何设施中有嗜肺军团菌增殖的风险

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Reclamation of Ampicillin Sensitivity for the Genetic Manipulation of Legionella pneumophila

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