首页> 外文期刊>Applied and Environmental Microbiology >Comparative study of a DNA hybridization method and two isolation procedures for detection of Yersinia enterocolitica O:3 in naturally contaminated pork products.
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Comparative study of a DNA hybridization method and two isolation procedures for detection of Yersinia enterocolitica O:3 in naturally contaminated pork products.

机译:DNA杂交方法和两种分离程序在天然污染猪肉产品中检测小肠结肠炎耶尔森菌O:3的比较研究。

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摘要

We compared a DNA-DNA hybridization assay, using a synthetically produced oligonucleotide probe, and two conventional isolation procedures (methods A and B) with regard to their relative efficiency in detecting Yersinia enterocolitica O:3 in naturally contaminated pork products. Method A was as described by Wauters et al. (Appl. Environ. Microbiol. 54:851-854, 1988). Method B has been recommended by the Nordic Committee on Food Analysis (method no. 117, 1987). The genetic probe was used in a colony hybridization assay to detect virulent yersiniae at each of the isolation steps with composed methods A and B. A total of 50 samples of raw pork products obtained from 13 meat-processing factories in Norway were examined. Y. enterocolitica serogroup O:3, biovar 4, was isolated from altogether 9 (18.0%) of the samples by using the two isolation procedures. In contrast, colony hybridization using the genetic probe indicated that 30 (60.0%) of the samples contained virulent yersiniae. All samples which were positive on cultivation were also positive by hybridization. The results indicate that the occurrence of pathogenic Y. enterocolitica in Norwegian pork products is substantially higher than previously demonstrated and, therefore, reinforce our suggestion that pork products represent an important potential source of human infection in Norway. The results also indicate that the use of conventional isolation procedures may lead to considerable underestimation of pathogenic Y. enterocolitica in pork products.
机译:我们比较了使用人工合成的寡核苷酸探针进行的DNA-DNA杂交测定和两种常规分离程序(方法A和B),它们在自然污染的猪肉产品中检测小肠结肠炎耶尔森菌O:3的相对效率。方法A如Wauuters等人所述。 (Appl.Environ.Microbiol.54:851-854,1988)。北欧食品分析委员会(方法117,1987)推荐了方法B。将该基因探针用于菌落杂交测定中,用组成的方法A和B在每个分离步骤中检测强毒耶尔森氏菌。共检查了从挪威13家肉类加工厂获得的50个生猪肉产品样品。通过两种分离方法,共从9个样本(18.0%)中分离出肠球菌耶尔血清群O:3,biovar 4。相反,使用遗传探针进行的菌落杂交表明30个样品(60.0%)含有强毒耶尔森氏菌。所有培养阳性的样品通过杂交也都是阳性的。结果表明,挪威猪肉产品中的致病性小肠结肠炎耶尔森菌的发生率大大高于先前证实的水平,因此,我们的建议加强了猪肉产品代表挪威人类感染的重要潜在来源的建议。结果还表明,使用常规分离程序可能会导致猪肉产品中病原性小肠结肠炎耶尔森氏菌的低估。

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