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Method for Adenosine 5′-Triphosphate Measurement on Coke Waste Activated Sludge

机译:焦炭废料活性污泥中5'-三磷酸腺苷的测定方法

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摘要

Measurement of adenosine 5′-triphosphate (ATP) in coke waste activated sludge can provide a simple method for estimating the levels of viable microbes in the sludge. However, the presence of inhibitors such as phenol in the sludge interferes when the luciferin-luciferase method is used to measure ATP. These inhibiting substances can be removed from the sludge before extraction of ATP by washing the cells with dilute sodium dodecyl sulfate.
机译:焦炭废料活性污泥中腺苷5'-三磷酸(ATP)的测量可提供一种估算污泥中活菌水平的简单方法。但是,当使用萤光素-萤光素酶方法测量ATP时,污泥中抑制剂如苯酚的存在会干扰。这些抑制物质可以在提取ATP之前通过用稀十二烷基硫酸钠洗涤细胞来从污泥中去除。

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