Computer Technique for Identification of Chromatographic Peaks

摘要

A computer program has been developed to identify known peaks in a chromatogram from a computer-interfaced chromatograph. The focal point of the program is a normalization technique, which refers the elution volume of each sample peak encountered to the elution volumes of two reference peaks that bracket the peak of interest, as is done in certain gas chromatographic normalization techniques. The normalized elution volume is determined by dividing the difference between the elution volumes of the sample peak and the first (early-eluting) reference peak by the difference between the elution volumes of the two reference peaks. The normalized elution volumes thus obtained are compared to normalized elution volumes of known compounds stored in bulk memory. The compounds now being used as reference peaks in the chromatograms from the UV-Analyzer are hypoxanthine, uric acid, hippuric acid, and p -cresol.