A semiautomatic procedure for the direct estimation of both serum calcium and magnesium is described. The indicator used, Eriochrome blue SE (EBSE), has the advantage of stability and freedom from interference by naturally occurring substances. The procedure requires 3-5 min. per sample and is very reproducible. The values obtained agree with those obtained by back-titration of excess chelator and are consistently 0.3 mg./100 ml. less than those obtained by a standard oxalate precipitation method.
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机译:描述了一种直接估算血清钙和镁含量的半自动程序。所使用的指示剂,Eriochrome blue SE(EBSE)具有稳定性和不受天然物质干扰的优势。该过程需要3-5分钟。每个样品都非常可重复。获得的值与通过过量螯合剂反滴定获得的值一致,始终为0.3 mg./100 ml。小于通过标准草酸盐沉淀法获得的那些。
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