1. By increasing the concentration of hydrochloric acid, sulfanilic acid, and sodium nitrite in the van den Bergh reagent used for determining total serum bilirubin it is possible to increase the absorbance of azobilirubin and to accelerate its reaction time to less than 10 minutes. A modified reagent is proposed for the Malloy and Evelyn procedure.2. The effects of temperature, urea, and protein on the coupling of serum bilirubin are discussed.3. Hemolysis seriously affects the coupling of bilirubin. The decrease in azobilirubin production appears to vary directly with the amount of hemolysis. When the amount of hemolysis is constant, the decrease in azobilirubin production appears to vary inversely with the bilirubin concentration. The interfering effect of hemoglobin is unexplained.
展开▼
机译:1.通过增加用于测定血清总胆红素的van den Bergh试剂中盐酸,磺胺酸和亚硝酸钠的浓度,可以提高偶氮胆红素的吸收,并将其反应时间缩短至不到10分钟。提出了一种用于Malloy和Evelyn程序的改良试剂。2。讨论了温度,尿素和蛋白质对血清胆红素偶联的影响。3。溶血严重影响胆红素的偶联。偶氮胆红素产生的减少似乎与溶血量直接相关。当溶血量恒定时,偶氮胆红素产生的减少似乎与胆红素浓度成反比。血红蛋白的干扰作用无法解释。
展开▼