NMR Study of Effects of MgCl2 on the Structural and Dynamical Properties of Yeast Phenylalanyl tRNA

摘要

Solvent exchange rates of selected protons were measured by NMR saturation recovery method for yeast tRNAPhe, at temperature from 25 to 40∩, in the presence of 0.1 M NaCl and various low levels of added magnesium ion. The exchange rates in zero Mg2+ concentration indicate early melting of acceptor stem, D stem, and tertiary structure. Addition of magnesium ion stabilizes the entire D stem more effectively than any other secondary or tertiary interactions.