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首页> 外文期刊>BMC Genomics >Upregulation of genes encoding digestive enzymes and nutrient transporters in the digestive system of broiler chickens by dietary supplementation of fiber and inclusion of coarse particle size corn
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Upregulation of genes encoding digestive enzymes and nutrient transporters in the digestive system of broiler chickens by dietary supplementation of fiber and inclusion of coarse particle size corn

机译:通过添加膳食纤维和添加粗粒玉米,在肉鸡消化系统中编码消化酶和营养转运蛋白的基因上调

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Measures to improve bird performance have been sought due to the imminent phase out of in-feed antibiotics in poultry and continued demand for higher poultry feeding efficiency. Increasing grain particle size and dietary fibre may improve gizzard function, digestive efficiency and nutrient absorption. This study was conducted to evaluate the effect increased particle size of corn and inclusion of sugarcane bagasse (SB) on mRNA expression of genes encoding digestive enzymes and nutrient transporters in broilers. A total of 336?day-old Ross 308 males were assigned in a 2?×?2 factorial arrangement of treatments with corn particle size - coarse 3576?μm or fine 1113?μm geometric mean diameter, and SB - 0 or 2% inclusion. Feed conversion ratio (FCR), weight gain and feed intake were measured from d 0–10 and d 10–24. The relative gizzard weight and mRNA expression of genes encoding digestive enzymes and intestinal nutrient transporters were measured on d 24. During d 10–24, a particle size × SB interaction was observed for FCR (P?
机译:由于家禽中即将淘汰饲料中抗生素的使用以及对更高家禽饲养效率的持续需求,已经寻求了改善禽类性能的措施。增加谷物的粒度和膳食纤维可以改善g的功能,消化效率和营养吸收。进行这项研究来评估玉米颗粒尺寸的增加和甘蔗渣(SB)的添加对肉鸡消化酶和营养转运蛋白编码基因mRNA表达的影响。分配了336天的Ross 308雄性,以2××2析因布置方式分配了玉米粒径-粗3576μm或1113μm的几何平均直径,SB-0或2%夹杂物。在d 0-10和d 10-24之间测量饲料转化率(FCR),增重和采食量。在第24天测量izz的相对重量和编码消化酶和肠道营养转运蛋白的基因的mRNA表达。在第10-24天,观察到FCR的粒径×SB相互作用(P 0.01),其中家禽粗饲含2%SB的玉米粉(CC)的FCR低于不含SB的CC的FCR。在第24天,胃蛋白酶原A和C的表达均观察到了××SB相互作用(P 0.01),它们与FCR呈负相关。仅在饲喂CC的家禽中添加2%SB上调了胃蛋白酶原A和C。此外,2%SB还上调了胰腺淀粉酶(AMY2A)和肠阳离子氨基酸转运蛋白1(CAT1)。饮食中的CC上调了十二指肠氨基肽酶N(APN),空肠丙氨酸,丝氨酸,半胱氨酸和苏氨酸转运蛋白1(ASCT1)和回肠肽转运蛋白2(PepT2)。这些结果表明,SB和粗粒度均调节编码重要消化酶和营养转运蛋白的基因的表达,因此与禽类生产性能直接相关。这些发现为将来的肉鸡饲喂管理中膳食纤维和粒度的组合效应提供了见解。

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