Species Identification and Clarithromycin Susceptibility Testing of 278 Clinical Nontuberculosis Mycobacteria Isolates

摘要

Purpose of this paper is to analyze different species’ proportion of nontuberculosis mycobacteria (NTM) and susceptibility to clarithromycin of different species. 278 clinical NTM isolates were identified into species by using 16S rRNA,rpoBandhsp65. Then clarithromycin susceptibility testing against different species was done separately, using microplate Alamar Blue assay. Finally, resistance isolates’erm(41)ofM. abscessuswere sequenced in order to analyze mechanisms for clarithromycin resistant. In this test, 131 isolates (47%) belonged toM. aviumcomplex (MAC), and 70 isolates (25%) belonged toM. abscessus. Nearly all theM. abscessussubsp.abscessusresistant to clarithromycin had T28 inerm(41). However, all theM. abscessussubsp.abscessussusceptible to clarithromycin had C28 inerm(41). In this study, we find that MAC was the most common pathogens of NTM, and the second one wasM. abscessus. However,M. chelonei, M. fuerth, andM. gordonwere rare. Clarithromycin had a good inhibition activity against all the NTM species exceptM. abscessussubsp.abscessus. Theerm(41)genotype is of high relevance to clarithromycin resistance.