Evaluation ofCandidaColonization and Specific Humoral Responses againstCandida albicansin Patients with Atopic Dermatitis

摘要

The aim of this study was to assess the candidal colonization and specific humoral responses againstCandida albicansin patients with atopic dermatitis. One hundred patients with atopic dermatitis and 50 healthy individuals were enrolled in the study. Skin and oral specimens from all participants were cultured on CHROMagarCandidamedium. Isolated yeasts were identified by using the sequence of the D1/D2 domain of the 26S rRNA gene. ELISA was used for detection of IgM, IgA, and IgG antibodies againstC. albicansin sera of participants.Candidaspecies were isolated from the skin and oral cavity of 31% of the patients and 12% of the controls. There was no significant difference betweenCandidacolonization in patients and controls (P>0.05).Candida albicanswas isolated from the skin and oral cavity of 23% of the patients and 6% of the controls (P< 0.05). There were no significant differences between serum levels of IgM and IgA in patients and controls (P>0.05). Serum level of IgG was significantly lower in patients than in controls (P<0.05). Type ofCandidacolonization can change in patients with atopic dermatitis. In addition, these patients have abnormalities in the production of antibodies againstCandida albicansthat may have a role in the pathogenesis of atopic dermatitis.