Changes of respiratory function on urine volume, renal AQP1, and AQP2 in mice with bronchial asthma and effects of lung-diffusing herb Platycodon grandiflorus (JACQ.) A.DC.

摘要

ObjectiveTo observe the effect of respiratory-function changes on urinary output and expression of aquaporin (AQP) in kidney tissue in bronchial asthma (BA) model mice. To explore the correlation between the lung controlling breathing and the lung regulating the waterways, and observe regulation by the lung-diffusing herb platycodon root (Platycodon grandiflorus(JACQ.) A.DC.).MethodsForty-five healthy female Balb/c mice were divided randomly into normal, model and platycodon root groups. The BA model was replicated by complex sensitization and stimulation with ovalbumin (OVA). Changes in airway resistance were detected using an AniRes2005 system, and 24-hour urine output collected by metabolic cages. Histopathologic changes in the lung and kidney were observed by H&E staining. Expression of the mRNA of AQP1 and AQP2 was detected by reverse transcription-polymerase chain reaction, immunohistochemistry, and immunofluorescence.ResultsCompared with the normal group, airway resistance in the inspiratory phase intensified in the model group (P?