Anti-cancer activity of an ethyl-acetate extract of the fruits of Terminalia bellerica (Gaertn.) Roxb. through an apoptotic signaling pathway in?vitro

摘要

ObjectiveTo investigate the biochemical constituents of the fruits ofTerminalia bellerica(Gaertn.) Roxb. (hereafter termedT. bellerica) and estimate the anti-cancer activity of different polar extracts.MethodsTo rapidly screen and identify the biochemical constituents of ethyl acetate (EA) extracts ofT. bellerica, ultra performance liquid chromatography-electrospray ionization/mass spectrometry (UPLC-ESI-MSn) was done. The CellTiter-Blue? cell-viability assay was used to ascertain the anti-cancer activity of different polar extracts in 10 human cancer cell lines.ResultsForty polyphenols of the EA extract ofT. bellericawere characterized tentatively. The EA extract exhibited significant anti-cancer activity against ZR-75-1?cells (half-maximal inhibitory concentration?=?27.33 (0.98) μg/mL) and Colo-205?cells (39.65 (2.99) μg/mL)in?vitro. Treatment of ZR-75-1?cells with 20 and 60?μg/mL of the EA extract elicited dose-dependent apoptosis percentages at an early stage of 17.58 (0.74) % and at a late stage of 29.20 (1.22) %; Colo-205?cells at the same concentration of EA extract had values of 21.33 (1.03) % and 40.55 (0.34) %, respectively. Western blotting suggested that ZR-75-1 and Colo-205?cells treated with the EA extract showed a similar increasing tendency for expression of cleaved anti-poly adenosine diphosphate ribose polymerase I.ConclusionWe identified a total of 40 chemical constituents, of which 11 were first obtained?from theTerminalia Linn.genus using UPLC-ESI-MSn. Meanwhile, we observe that the EA extract ofT. bellericapossesses anti-cancer activity, especially against breast and colon cancers.