Evaluation of α-amylase, lipase inhibition and in-vivo pharmacological activities of Eucalyptus camaladulensis Dehnh leaf extract

摘要

In this present study, phytochemical screening, anti-ulcer assay, anti-diarrhea assay, anti-inflammatory assay, analgesic assay, lipase activity assay, amylase activity assay and the anti-bacterial activity ofEucalyptus camaladulensisDehnh leaf extracted with methanol and 50% ethanol was analyzed for biological significance. Physical characterization of the non-volatile component revealed the higher yield of 16.92% in 50% ethanol expediting the use of 50% ethanol as a better alternative. Further use of crude extract revealed 33.89% (IC50?=?1.44?mg/ml) of α-amylase inhibition by methanol extract and 33.87% (IC50?=?3.21?mg/ml) lipase inhibition by 50% ethanol extract. Furthermore, 44.44% protective ratio towards ulcer was observed with the methanol extract, whereas 54.58% anti-inflammatory activity was shown by the 50% ethanol extract. The effectiveness of the extract was further enhanced by the presence of 62.54% motility and best analgesic property at 180?min of the exposure of the extract orally. The antioxidant activity of crude methanol extract revealed an IC50value 601.8?μg/ml whereas, ethanol extract showed 1279.58?μg/ml in DPPH assay. Result revealed several health benefits ofE.?camaldulensisDehnh leaf.