A SENSITIVE SEQUENTIAL INJECTION ANALYSIS (SIA) DETERMINATION OF MEMANTINE HYDROCHLORIDE USING LUMINOL-HYDROGEN PEROXIDE INDUCED CHEMILUMINESCENCE DETECTION

摘要

A fully automated sequential injection analysis (SIA) method for chemiluminescence (CL) determination of Al-zheimer's drug memantine hydrochloride was developed and presented. The employing of SIA injection analysis facilitates fluidic handling and lowering the consumption of sample and reagents. The basic CL reaction was based on the emission of CL radiation due to the reaction of luminol with hydrogen peroxide in basic medium. The CL emission has been monitored using FIAlab system. The optimum conditions and characterizations were optimized using a computer-aided simplex method. Under the optimal conditions the linear calibration graph was obtained within the range of 0.0001-1.0 μg mL-1, (r=0.9996) with detection limit of 3.3x10-5 μg mL-1 along with relative standard deviation 1.8 % (n). The flow rates were 120 μL s-1, for the aspiration into the holding coil and 80 μL s-1 for detection. The sample frequency throughput was 103 h-1. The proposed CL method was successfully applied for determination of the tested drug in pure form and its pharmaceutical formulations. The interference of some common additive compounds such as glucose, lactose, starch, talc and magnesium stearate was investigated. No interference was recorded. The obtained SIA results were statistically compared with those obtained from a reported method and did not show any significant difference.