Anther-derived callus induction based on culture medium, myo-inositol, AgNO3 and Fe-EDTA in Seolhyang strawberries

摘要

Haploid breeding is an effective method of shortening the breeding period of plants. In this study, to develop a haploid breeding technique, optimum conditions of the culture medium were investigated for anther-derived callus induction in the strawberry cultivar Seolhyang. The effects of the culture medium type, myo-inositol, auxin and cytokinin combination treatment, silver nitrate (AgNO3), and ferric ethylenediaminetetraacetic acid (Fe-EDTA) on anther-derived callus induction were analyzed. Anthers were incubated in Murashige and Skoog (MS) medium, Gamborg B5 medium (B5), and Lichter medium (NLN) for 8 weeks. Each culture medium had 0.4 mg?L–1 of 6-benzyladenine (BA), 0.1 mg?L?1 of indole-3 acetic acid (IAA), and 2.0 mg?L?1 of 2,4-dichlorophenoxyacetic acid (2,4-D) added to it. Results showed that MS medium was most effective in callus induction. When 100 mg?L?1 of myo-inositol was added to each medium, the callus induction rate increased. Auxin and cytokinin combination treatment was more effective with the addition of 0.4 mg?L?1 of BA, 0.1 mg?L?1 of IAA, and 2.0 mg?L?1 of 2,4-D to MS medium compared to the addition of 0.1 mg?L?1 of BA, 2.0 mg?L?1 of IAA, and 0.4 mg?L?1 of 2,4-D. With AgNO3 treatment, the highest callus induction rate was found at a concentration of 25 mg?L?1. The callus induction rate increased as the AgNO3 concentration increased; however, a significant decrease was seen at 30 mg?L?1. In the case of Fe-EDTA, the most effective concentration for callus induction was 25 mg?L?1. Therefore, supplementing myo-inositol, AgNO3 and Fe-EDTA can help in anther-derived callus induction in Seolhyang strawberries.