Clinical Variation ofPlasmodium falciparum eba-175,ama-1, andmsp-3Genotypes in Young Children Living in a Seasonally High Malaria Transmission Setting in Burkina Faso

摘要

The association betweenP. falciparum eba-175,ama-1, andmsp-3polymorphism in the pathogenicity of malaria disease was investigated. We therefore compared the prevalence of different alleles between symptomatic and asymptomatic malarial children under five years of age living in Burkina Faso. Blood filter papers were collected during the 2008 malaria transmission season from 228 symptomatic and 199 asymptomatic children under five years of age. All patients were living in the rural area of Saponé at about 50 km from Ouagadougou, the capital city of Burkina Faso.P. falciparumparasite DNA was extracted using QIAGEN kits and the alleles diversity was assessed by a nested PCR. PCR products were then digested by restriction enzymes based on already described polymorphic regions of theeba-175,ama-1, andmsp-3genes. The individual alleleseba-175_FCR3andmsp-3_K1frequencies were statistically higher (p<0.0001) in the asymptomatic group compared to the symptomatic ones. No statistically significant difference was noted in the prevalence ofama-1-3D7,ama-1-K1, andama-1-HB3 genotypes between the two groups (p>0.05). The comparative analysis ofP. falciparumgenotypes indicated that the polymorphism ineba-175andmsp-3genotypes varied between asymptomatic and symptomatic clinical groups and may contribute to the pathogenesis of malaria.