Calibration of multi-channel pipettes using gravimetric method in accordance with the ISO 8655-6

摘要

A calibration system was set up for calibrating multi-channel pipettes using the gravimetric method in accordance with the ISO 8655-6. The calibration system consisted of a display unit, a motor control unit and a measuring unit. The measuring unit included a single weighing cell, twelve individual containers for receiving the dispensed volume from multi-channel pipettes and transport rails to automatically convey the containers to the weighing cell one by one. This paper described the measurement traceability for volume measurements to the SI unit of mass by using the calibration system and the validation of the calibration system for volume measurements. The calibration system was shown to be capable of measuring the dispensed volume from 30 μl to 1000 μl with the measurement uncertainty of 0.7 μl to 2.9 μl.