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Study of Compatibility Relationships Among Some Almond Cultivars and Genotypes Using of SAlleles Identification

机译:利用SAlleles鉴定研究某些杏仁品种与基因型之间的相容性关系

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Almond (Prunus dulcis L.) is one of the most important nut crops in Iran. Most almond cultivars and genotypes are self-incompatible. However, research on S-alleles indicates that it is very efficient in cultivar selection. Selfincompatibility in almond is gametophytic and controlled by a single S-locus with multiple codominant alleles. In this study, compatibility relationships among cultivars, “Tuono”, “Shokofeh”, “Sahand” and five improved genotypes “A1.16”, “A9.7”, “A8.39”,“A10.11” and “A230,” was investigated by the PCR of S-alleles. Degenerate primers (PaConsI-F, EMPC1consRD, EM-PC2consFD, and EM-PC3consRD) were used for amplification of S-alleles. Results showed that only “A10.11” and “A8.39” were completely cross-incompatible, but all of the other studied cultivars and genotypes were crosscompatible. Furthermore, cultivar “Tuono” and genotype “A1.16” had a self-fertility allele.
机译:杏仁(Prunus dulcis L.)是伊朗最重要的坚果作物之一。大多数杏仁品种和基因型是自我不相容的。然而,对S等位基因的研究表明它在品种选择中非常有效。杏仁中的自我不相容性是配子体性的,并且由具有多个共性等位基因的单个S基因座控制。在这项研究中,品种“ Tuono”,“ Shokofeh”,“ Sahand”和五个改良基因型“ A1.16”,“ A9.7”,“ A8.39”,“ A10.11”和“ A230”之间的相容性关系,通过S-等位基因的PCR研究。简并引物(PaConsI-F,EMPC1consRD,EM-PC2consFD和EM-PC3consRD)用于扩增S等位基因。结果表明,只有“ A10.11”和“ A8.39”是完全交叉不兼容的,但是所有其他研究的品种和基因型都是交叉兼容的。此外,品种“ Tuono”和基因型“ A1.16”具有自育等位基因。

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