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Pulp and plaque microbiotas of children with severe early childhood caries

机译:患有严重幼儿龋齿的儿童的纸浆和菌斑微生物区系

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Background and objective Bacterial invasion into pulps of primary teeth can lead to infection and premature tooth loss in children. This pilot study aimed to explore whether the microbiota of carious exposures of dental pulps resembles that of carious dentin or that of infected root canals. Design Children with severe early childhood caries were studied. Children were consented and extent of caries, plaque, and gingivitis measured. Bacteria were sampled from carious lesion biofilms and vital carious exposures of pulps, and processed by anaerobic culture. Isolates were characterized from partial sequences of the 16S rRNA gene and identified by comparison with taxa in the Human Oral Microbiome Database ( http://www.HOMD.org ). The microbiotas of carious lesions and dental pulps were compared using univariate and multivariate approaches. Results The microbiota of cariously exposed pulps was similar in composition to that of carious lesion biofilms except that fewer species/taxa were identified from pulps. The major taxa identified belonged to the phyla Firmicutes (mainly streptococci) and Actinobacteria (mainly Actinomyces species). Actinomyces and Selenomonas species were associated with carious lesions whereas Veillonella species, particularly Veillonella dispar was associated with pulps. Other bacteria detected in pulps included Streptococcus mutans , Parascardovia denticolens , Bifidobacterium longum , and several Lactobacillus and Actinomyces species. By principal, component analysis pulp microbiotas grouped together, whereas those in caries biofilms were widely dispersed. Conclusions We conclude that the microbiota of cariously exposed vital primary pulps is composed of a subset of species associated with carious lesions. Vital primary pulps had a dominant Firmicutes and Actinobacteria microbiota which contrasts with reports of endodontic infections which can harbor a gram-negative microbiota. The microbiota of exposed primary pulps may provide insight into bacterial species at the forefront of caries invasion in dentinal lesions that can invade into the pulp and the nature of species that need suppressing for successful pulp therapy.
机译:背景和目的细菌侵入乳牙的牙髓会导致儿童感染和牙齿早脱落。这项初步研究旨在探讨龋牙暴露的微生物区系与龋牙本质或感染的根管相似。设计研究了患有严重幼儿龋齿的儿童。征得儿童的同意,并测量龋齿,斑块和牙龈炎的程度。从龋病病变生物膜和牙髓重要龋病暴露中取样细菌,并通过厌氧培养进行处理。从16S rRNA基因的部分序列中鉴定出分离物,并通过与人类口腔微生物组数据库(http://www.HOMD.org)中的分类单元进行比较进行鉴定。使用单变量和多变量方法比较龋齿病变和牙髓的微生物群。结果龋牙暴露的牙髓的微生物群与龋病灶生物膜的微生物群相似,但从牙髓中鉴定出较少的种类/分类。鉴定出的主要分类群属于菌门类(主要是链球菌)和放线菌(主要是放线菌属)。放线菌属和硒单胞菌属物种与龋齿病变有关,而Veillonella物种,尤其是dispar Veillonella Dispar与牙髓有关。纸浆中检测到的其他细菌包括变形链球菌,齿状副鞭毛虫,长双歧杆菌以及几种乳杆菌和放线菌。从原理上讲,成分分析纸浆微生物群聚在一起,而龋齿生物膜中的微生物群则广泛分布。结论我们得出的结论是,龋齿暴露的重要原浆的微生物群是由与龋齿病变相关的物种组成的。重要的原浆具有主要的菌毛和放线菌菌群,这与牙髓感染可携带革兰氏阴性菌群的报道形成对比。裸露的原浆的微生物区系可以洞悉可能侵入牙髓的龋齿侵入龋齿最前沿的细菌种类以及成功进行牙髓治疗需要抑制的菌种的性质。

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