Authentication of Taxillus chinensis using DNA barcoding technique

摘要

The purpose of this study is to evaluate the five candidate DNA barcodes of?rbcL,matK,?psbA-trnH, ITS and ITS2 in order to distinguish?Taxillus chinensis?from adulterants. Of the five barcodes, the amplification and sequencing efficiencies ofrbcL?and?trnH-psbA?were?100%. Meanwhile, the efficiency of?matK, ITS and ITS2 regions was very low due to failure in PCR amplification or sequencing. The?psbA-trnH?exhibited high inter-specific divergence and distinguished?T. chinensis?from other parasitic loranthus. The?rbcL?gene demonstrated lower inter-specific divergence and identification efficiency. In conclusion,?psbA-trnH?is a suitable DNA barcode in authenticating?T. chinensis?and its related parasitic loranthus.