A detailed chemical composition and antimicrobial activity of Hypericum richeri Vill. subsp. grisebachii (Boiss.) Nyman essential oil from Serbia

摘要

The essential oil of fresh aerial parts of?Hypericum richeri?subsp.?grisebachii?obtained by hydrodistillation was analyzed by?gas chromatography (GC) and?gas chromatography/mass spectral?GC/MS. One hundred and five constituents identified accounted for 96.1% of the total oil. The major components of the oil were:?germacrene D (13.1%), dodecanal (11.9%), β-caryophyllene (7.4%), β-pinene (5.8%), caryophyllene oxide (4.2%) and (E)-β-ocimene (3.3%). The volatile profile of?H. richeri?subsp.?grisebachii?was characterized by a large amount of sesquiterpenoids (56.2%), especially by hydrocarbons of this fraction (34.1%). The?in vitroantimicrobial activity of essential oil was also examined against a panel of microbial strains by broth microdilution assay and it was found to have moderate effect against all tested microorganisms.