In vitro anti-angiogenic activity fractions from hydroalcoholic extract of Elaeagnus angustifolia L. flower and Nepeta crispa L. arial part

摘要

Angiogenesis is an essential event in the tumor growth and Metastasis. The aim of our research is to study the effect of?Elaeagnus angustifolia?and?Nepeta crispa?extracts on anti-angiogenic activities in human umbilical endothelial cells (HUVEC). Hydroalcoholic extract and its?successive hexane, ethyl acetate, chloroform and aqueous fractions?were used in different concentration by three dimensional cytodex-collagen model. Hydroalcoholic extracts of?E. angustifolia?flower in 200 μg/ml and?N. crispa?aerial part in 400 μg/ml potentialy inhibited angiogenesis activity of HUVEC and 10 μg/ml both of ethyl acetate and chloroform fractions exerted prevention of this activity. Therefore,?E. angustifolia?flower andN. crispa?aerial part could be candidate for therapeutic or preventive activity against angiogenesis related disorders.