Antiviral activity of leaves extracts of Marrubium alysson L.

摘要

Petroleum ether, ethyl acetate, chloroform, butanol and methanol extracts of the leaves ofMarrubium alysson L. were screened for?in vitro?antiviral activity against human cytomegalovirus (HCMV) strain AD-169 (ATCC Ref. VR 538) and coxsackie B virus type 3 (CoxB-3) using a cytopathic effect (CPE) reduction assay. Total phenolic content of the extracts were determined by the Folin-Ciocalteu colorimetric method. The most potent inhibition was observed with methanol extract, which inhibited HCMV and Cox B3 viruses replication at 100 μg/ml without showing cytotoxic effects. Good activities were also found with the ethyl acetate, butanol and chloroform extracts which exhibited antiviral effect against both HCMV and Cox B3 at 100 to 250 μg/ml.