Healing Effect of Conditioned Media from Bone Marrow-Derived Stem Cells in Thioacetamide-induced Liver Fibrosis of Rat

摘要

Following repeated injury, the liver undergoes tissue remodeling and fibrosis. This study investigated the healing effect of conditioned media (secreta) from rat bone marrow-derived stem cells (BMSCs) in thioacetamide (TA)-induced liver fibrosis rat model. During 2014, thirty male Wistar rats were randomly allocated into two groups of sham control and treatment. The rats of sham control group were subdivided into three subgroups and were sampled on weeks 14, 18 and 20 after fibrosis induction. The rats of treatment group were subdivided into two subgroups and were sampled on weeks 4 and 6 after treatment by conditioned media. Fibrosis was induced by intraperitoneal administration of TA. Animals were valuated histologically and for liver function tests after TA-induced fibrosis and injection of conditioned media. Conditioned media were provided from the supernatant of BMSCs culture flasks. Administration of TA resulted into infiltration of inflammatory cells and deposition of collagen fibers in hepatic tissue. A significant healing was noticed in liver four and six weeks after post-injection of BMSCs conditioned media. Regarding fibrosis and necrosis, a significant reduction appeared 4 and 6 weeks after BMSCs conditioned media therapy. Aspartate aminotransferase (AST) and alanine transaminase (ALT) levels decreased 4 and 6 weeks after BMSCs conditioned media therapy. Regarding alkaline phosphatase (ALP) and albumin levels 4 and 6 weeks after BMSCs conditioned media therapy, a non-significant increase was seen. This study indicated that the BMSCs conditioned media had a positive healing effect in rearrangement of hepatic fibrosis. So it can be an alternative therapeutic choice to ameliorate liver fibrosis.