The Effects of Kolaviron (Methanolic Extract of Garcinia kola Seeds) on the Histoarchitectural Studies of the Hypothalamo-pituitary-gonadal Axis in Female Wistar Rats

摘要

Kolaviron is known for its anti-inflammatory property whereas ovulation occurs through inflammatory process; this work therefore, determined its relationship to infertility. A total of thirty adult female Wistar rats were used for this experiment. The animals were randomly divided into three groups: A, B and C with ten animals in each group. Extracts were administered to the animals orally for 14 days. Group A (control) were given distilled water; Group B were given kolaviron at concentration of 200 mg kg^-1 b.wt. once daily and Group C were given kolaviron at concentration of 100 mg kg^-1 twice daily. At the end of the experimental period, the animals were anaesthesized with chloroform inhalation. The hypothalamus, anterior pituitary and ovary were removed, weighed and fixed in 10% formol calcium for Haematoxylin and Eosin staining, demonstration of Nissl substance in hypothalamus and demonstration of special stain using Aldehyde Fuschin Thionin for delta cells of anterior pituitary gland. The results showed that Relative brain weight (F = 12.348, p<0.05) and relative gonads weight (F = 10.700, p<0.05) were significantly difference across the groups. The histoarchitechture of the ovary showed that kolaviron ingestion by the female wistar rats revealed no growing follicles which were obvious in control group, the dump-bell shapes of the delta cells in the anterior pituitary gland were normal in group A but distorted in groups B and C while kolaviron administration showed no effect on the hypothalamus. In conclusion, kolaviron altered the histoarchitecture of hypothalamo-pituitary-gonadal axis which could cause infertility in female wistar rats .