In order to better use the antagonistic actinomycetes strain F22-29, the formula of the seed coating with the fermentation broth of F22-29 strain was screened and optimized by two orthogonal designed tests. The results showed that the best formula of seed coating agent was 1% sodium lignin sulfonate: 2% carboxy methylcellulose sodium: 2% dextrin. Compared with the root irrigation of the fermentation broth, the control efficiency of the seed coating against Rape Plasmodiophora brassicae was increased by 38.11%, and that of the seed coating against the Clubroot Disease in Brassica napus was increased by 63.16%. For Chinese cabbage coated seed, the control efficiency was increased by 15.78%.
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