Apoptosis Induction (Caspase-3,-9) and Human Tongue Squamous Cell Carcinoma VEGF Angiogenesis Inhibition using Flavonoid’s Ethyl Acetate Fraction of Papua Ant Hill (Myrmecodia pendans) SP-C1

摘要

Human tongue squamous cell carcinoma was casued by inability to control cell survival and to control cel motility. Apoptosis is a normal component process of multicellular organism’s development and health. Apoptosis is a programmed cell death as a response to various stimuli. Angiogenesis or neovascularisation is a basic developing sign in cell physiology and pathology. This research was to identify flavonoid’s ethyl acetate fraction from Anthill (Myrmecodia pendans) which has a potential as a anti-cancer to SP-C1 cell. This research was to analyze the Anthill flavonoid’s ethyle acetate as an anti cancer to apoptosis induction (caspase-3 and -9 analyses) to Supri’s Clone (SP-C1) tongue cancer cell and to analyze angiogenesis inhibition by VEGF protein expression. The data from this research was analyzed using Two Way ANOVA and t test (Tukey’s test) with confidence interval 95%. Caspase-3 and -9 colorimetric analyses showed increasing SP-C1 cell inhibition by time and concentration. ELISA analysis showed increasing proteolytic activity on caspase-9 compared with caspase-3. Increasing proteolytic activity with the increasing concentration from 5 μg/ml to 100 μg/ml could also be observed. VEGF angiogenesis inhibition showed increased flavonoid concentration was followed by increased VEGF expression. Otherwise, SP-C1 cell (control) did not show any VEGF expression. Anthill’s flavonoid ethyl acetate fraction (Myrmecodia pendans) had anti-tumor activity on several molecular targets from apoptosis and VEGF angiogenesis of tongue squamous cell carcinoma.