Six probiotic candidates were grown overnight at 37 °C in De Man Rogosa Sharpe (MRS) broth supplemented with 0.4 mg/mL of linoleic acid. The concentrations of the metabolite products in the culture media were determined using GC-MS after extraction and direct transesterification of the fatty acids. Linoleic acid was added to the sample as an internal standard. The result showed that Lactobacilluscasei strainAPwas able to convertfreelinoleicacidto conjugated linoleic acid in themedia more than 60% while five other potential probiotics such as Lactobacillus casei AF, Lactobacillus casei AG, Pediococcus acidilactici AA, Pediococcus acidilactici BE and Pediococcus acidilactici BK have abilities to convert free linoleic acid to palmitic acid, stearic acid and oleic acid with varying percentages.
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机译:六种益生菌候选物在补充了0.4 mg / mL亚油酸的De Man Rogosa Sharpe(MRS)肉汤中于37°C过夜生长。脂肪酸的提取和直接酯交换反应后,使用GC-MS测定培养基中代谢产物的浓度。将亚油酸作为内标添加到样品中。结果表明,干酪乳杆菌菌株AP能够在60%以上的培养基中将游离亚油酸转化为共轭亚油酸,而其他5种潜在的益生菌如干酪乳杆菌AF,干酪乳杆菌AG,嗜酸乳球菌AA,嗜酸乳球菌B则具有抗乳酸菌能力酸到棕榈酸,硬脂酸和油酸的百分比不同。
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