Correlation of EPO and VEGF levels in serum and aqueous humor with ocular hemodynamics and oxidative stress - mitochondrial function in patients with glaucoma

摘要

To study the correlation of EPO and VEGF levels in serum and aqueous humor withocular hemodynamics and oxidative stress - mitochondrial function in patients with neovascularglaucoma (NVG). Methods: A total of 47 patients who were diagnosed with neovascularglaucoma in Mianyang Wanjiang Ophthalmic Hospital between April 2015 and April 2017were selected as the NVG group of the study, and 75 patients who were diagnosed withcataract in Mianyang Wanjiang Ophthalmic Hospital over the same period were selected as theCAT group of the study. The aqueous humor and serum samples were collected to determinethe contents of EPO, VEGF, oxidative stress molecules and mitochondrial function molecules;the intraocular vascular hemodynamic parameters were detected. Results: EPO and VEGFcontents in serum as well as EPO and VEGF contents in aqueous humor of NVG group weresignificantly higher than those of CAT group; EDV and PSA levels of central retinal artery aswell as EDV and PSA levels of short posterior ciliary artery in NVG group were significantlylower than those in CAT group and negatively correlated with EPO and VEGF contents inaqueous humor; MDA, AOPP, Drp1, CytC, Apaf-1 and caspase-3 contents in aqueous humorof NVG group were significantly higher than those of CAT group and positively correlatedwith EPO and VEGF contents in aqueous humor while SOD, GSH-Px, CAT, Mfn1 and Mfn2contents were significantly lower than those of CAT group and negatively correlated withEPO and VEGF contents in aqueous humor. Conclusion: The abnormal increase of EPO andVEGF contents in serum and aqueous humor of patients with glaucoma is closely related to theincrease of ocular blood resistance, activation of oxidative stress reaction and mitochondrialdysfunction.