Mating system and seed variation of Acacia hybrid (A. mangium ?— A. auriculiformis)

摘要

The mating system and seed variation of Acacia hybrid (A. mangium ?— A. auriculiformis) were studied using allozymes and random amplified polymorphic DNA (RAPD) markers, respectively. Multi-locus outcrossing rate estimations indicated that the hybrid was predominantly outcrossed (mean?±s.e. $t_{m} = 0.86pm 0.01$). Seed variation was investigated using 35 polymorphic RAPD fragments. An analysis of molecular variance (AMOVA) revealed the highest genetic variation among seeds within a pod (66%a€“70%), followed by among pods within inflorescence (29%a€“37%), and the least variation among inflorescences within tree ( 1%). In addition, two to four RAPD profiles could be detected among seeds within pod. Therefore, the results suggest that a maximum of four seeds per pod could be sampled for the establishment of a mapping population for further studies.