Protein mapping by combined 2-D electrophoresis and mass spectrometry: an approach to identify the proteome changes in muscle of diabetic rats and treatment with Cynodon dactylon L

摘要

Objective: A proteomics approach used to understand the diabetes mellitus-related changes in muscle proteome composition during diabetes mellitus and treatment with Cynodon dactylon. This approach may provide way to investigate the muscle function associated with diabetes and metabolic protein mechanism responsible for this disease and also new targets of diabetes diagnosis and effective treatment. Methods: We used comparative proteomic techniques to identify muscle proteins associated with diabetes mellitus in alloxan-induced rats by using 2-dimensional electrophoresis (2-DE), matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and peptide mass fingerprint (PMF) analysis. The rats (four groups; six animals each group) were made diabetic by single intraperitoneal (i.p.) alloxan injection (150 mg/kg b.w). After 72 h alloxan injection, the treatment was with C.dactylon leaf extract for 15 days. The differential protein expressions were analyzed in alloxan-induced diabetic rats and those treated with C.dactylon leaf extract. Results: In this experiment, two proteins, i.e. fructose-bis-phosphate aldolase and glyceraldehyde-3-phosphate dehydrogenase were up-regulated in diabetic treated group. Conclusion: This result suggests that, up-regulated proteins were involved in glucose metabolism and also in transcriptional control of nuclear membrane fusion. These proteins were up-regulated by the effect of C.dactylon extract in muscle tissues playing a critical role in glucose metabolism which in turn control and reduce the severity of diabetes mellitus.