Effects of Sprint Training on Glutathione Expression in Liver and Skeletal Muscle

摘要

McCartney M, Keesee C, Fletcher L, Stover S. Effects of Sprint Training on Glutathione Expression in Liver and Skeletal Muscle. JEPonline 2011;14(5):66-74. Acute anaerobic exercise promotes oxidative stress. However, previous studies indicate that high intensity sprint training can attenuate the effects of oxidative stress. It was hypothesized that an upregulation of reduced glutathione (GSH) is responsible for the protection against oxidative stress associated with sprint training. Thirty-three mice were randomly divided into three groups: control, acute, and trained. Trained mice participated in a high intensity exercise program consisting of treadmill running two days a week for 12 weeks. At the end of the training period, trained and acute mice engaged in a single session of intense sprinting. Control mice did not exercise. Concentrations of GSH and oxidized glutathione (GSSG) were determined spectrophotometrically in the soleus and extensor digitorum longus (EDL) muscles, as well as the liver. Following acute exercise, GSH concentrations (mmol/g tissue) in the soleus and EDL decreased significantly, relative to their respective controls. At the same time, GSSG concentrations (mmol/g tissue) in both muscles increased significantly. After 12 weeks of sprint training, GSH concentrations in the soleus and EDL were not significantly different from their respective controls, even as GSSG concentrations in the muscles increased significantly. Results of the study failed to demonstrate any change in liver glutathione after acute sprinting or sprint training. Data indicate that control levels of GSH can be recovered by skeletal muscle as a result of high intensity sprint training. However, the upregulated GSH does not appear to be imported from the liver.