Optimization and Interactive Effects of Salinity, Fertilizer and Inoculums Concentration on Biodegradation in Marine Environment

摘要

With the speeding up of the exploitation and shipments of petroleum, the oil spill or off-shore oil production all over the world have dramatically?increased. Bioremediation has been recognized as a cost-effective and environment-friendly approach for cleanup of the petroleum pollutants from marine environment. Though some studies have reported the effect of every factor for the biodegradation, there are few reports on the interactive and cumulative effect of environment factors on biodegradation. In the paper, interactive effects of salinity, fertilizer and inoculums concentrations on biodegradation were studied by response surface methodology, in order to provide better theoretical basis for use the biodegradation in the marine environment. The results indicated that there was the good agreement between the predicted values and the experimental values. Further analyzing the variance (ANOVA),?the value of determination?coefficient (R 2 =0.9831) indicated that the total variations?for the model was negligible. And, the second-order polynomial equation was defined. Additionally, residual normal, predicted value vs. actual value, residual value vs. predicted value were also analyzed and the results?were shown that the model was effective.?Lastly, according to the interactive effects of three factors and p-values, the factors were in several groups by descending order: salinity ＞ inoculums concentration ?≈ nitrogen and phosphorus ratio.