Functional denture soft liner with antimicrobial and antibiofilm properties

摘要

Background/purposeDenture soft liners, especially used for elders who have poor disinfection habits, provide a favourable environment for accumulation and colonization of microorganisms. Thisin?vitrostudy is aimed to investigate the effectiveness of natural carvacrol incorporation into soft lining material on the inhibition of oral pathogens.Materials and methodsAntimicrobial susceptibility of carvacrol was primarily determined by disc diffusion method. Soft lining material was prepared as recommended by the manufacturer and 10?μL carvacrol was added aseptically to the soft liner discs. Inhibition zones for the control discs without carvacrol (C) and carvacrol-incorporated discs (CL) were determined by disc diffusion method. The biofilm inhibition percentages of carvacrol on soft liner was determined by MTT assay and also observed by Scanning Electron Microscopy (SEM).ResultsCarvacrol displayed great antimicrobial activity for yeast, Gram-negative and Gram-positive strains. The highest inhibition zone of carvacrol (41.33?±?1.53?mm) was measured forBacillus subtilisstrain which is followed byCandida albicansandStreptococcus sanguis(34.00?±?1.73?mm and 32.33?±?0.58?mm, respectively). The inhibition zones were also similar for soft liner discs with carvacrol, with the highest inhibition zones againstB. subtilis,Streptococcus mutansandC. albicans(43.67?±?0.58?mm, 40.33?±?0.58?mm and 38.33?±?1.15?mm, respectively). Incorporation of carvacrol into the soft liner decreased (98.03?±?0.2%) of the biofilm formation forC. albicans.ConclusionCarvacrol-incorporation obviously decreased the colonization and plaque formation of oral pathogens, especiallyC. albicansaccumulation. Carvacrol may be useful as a promising agent for antibacterial and antifungal management for denture soft lining materials.