A study of Analysis of Endothelial Progenitor cells in Peripheral blood in patients with coronary artery disease

摘要

Objective: The study is aimed to analyze endothelial progenitor cells (EPCs) in coronary artery disease and also to identify predictive marker to assess EPCs in the same condition. Background: Endothelial progenitor cells (EPC) predict morbidity and mortality in cardiovascular risk. The ideal way to cure atherosclerosis and the subsequent end organ damage is to restore and rejuvenate the dysfunctional vasculature by induction of EPCs. Methods and Results: EPCs characterized by CD34 and KDR or CD133 markers still in progress to standardize. Hence, current study demonstrates the marker to identify the EPC in peripheral blood of patients with CAD. Flow cytometry used to quantify EPCs in 25 coronary artery disease as confirmed on angiography patients and 25 control subjects without CAD were prospectively enrolled in the study. To identify predictive EPCs in the peripheral blood, we used various combinations of markers like CD34+KDR+CD133+CD45-. We found that CAD patients presented significantly lower levels of EPCs, which expresses the markers CD34+CD45-KDR+ and CD133+CD45-KDR. EPCs which express CD34+CD45-KDR+subpopulation shows higher significant change between CAD and control group (p<0.001). Conclusion: In conclusion, study indicates that there is a progressive decrease in EPCs levels in CAD patients. Patients with low EPC counts had a higher incidence for cardiovascular events. The level of circulating EPCs with CD34+CD45-KDR+ expression predicts the occurrence of cardiovascular events and may help to identify patients at increased cardiovascular risk.