Multiplica??o, alongamento e enraizamento de brota??es in vitro de clones de cana-de-a?úcar submetidos a diferentes concentra??es de 6-benzilaminopurina e ácido giberélico

摘要

The culture of meristems allows obtaining plant sugarcane healthy with genetic stability and enables the production of a large number of seedlings during the year. The aim of this work was to establish suitable conditions for seedling production in vitro by meristems of three potential clones sugarcane (RB036091, RB036152 and RB036066). The multiplication and elongation of shoot buds were evaluated. The BAP (0, 0.444, 0.888 and 1.776 mM) was added in the medium for multiplication of shoot buds and GA3 (0, 0.3 and 0.6 mM) for elongation of shoot buds. Presence of BAP in the medium had an inhibitory influence on shoot buds height. The BAP in the medium promoted the tillering for the three clones. The GA3 increased the height of shoots for clones RB036152 and RB036066. The GA3 promoted the formation of a greater plant, however, fewer roots were formed for all clones tested. It was concluded that the concentration of 0.444 mM BAP promotes the multiplication of clones RB036091, RB036066 and RB036052. The clone RB036091 showed the higher multiplication rate. The GA3 promotes the elongation of the seedlings for clones RB036152 and RB036066. Key-words: SaccharumL., micropropagation, meristems, seedling production.