In Vitro evaluation of native isolate of Metarhizium anisopliae (Metchinkoff) sorokin and its oil in water formulations against Odoiporus longicollis olivier

摘要

Banana pseudostem borer Odoiporus longicollis Olivier is one of the serious monophagous pest threatening the production and productivity of banana in south India. The native isolate of entomopathogenic fungi Metarhizium anisopliae (Metchinkoff) Sorokin was identified and evaluated for its virulence to O. longicollis compared with other entomopathogenic fungal isolates. The native isolate M. anisopliae (Ma-BW) showed higher virulence to adults with the minimum LC50 value ～1.0 × 107 spores ml-1 and faster lethal effect on adult O. longicollis with the shortest LT50 value of 62.54 hours. Among different oil in water formulations of Ma-BW evaluated against adult O. longicollis in laboratory at 2% showed that the formulation of Ma-BW in ground nut oil performed superior with adult mortality of 83.33 per cent at 120 HAT followed by oil formulations of Ma-BW in sunflower oil, gingelly oil and neem oil with76.67 per cent adult mortality at the same concentration and time period.