Establishment of effective regeneration system in Egyptian-adapted canola var. pactol

摘要

Canola (Brassica napus L.) is the oilseed crop of cold season countries, yet recently new varieties are being tested in warmer-climate countries. In Egypt, new varieties have been introduced / tested for adaptation to Egyptian environment, therefore gaining the interest of workers in the field of oil-crops production. In the current work, canola var. Pactol, a well adapted variety to Egyptian environment, was tested for regeneration ability via direct and indirect shoot organogenesis, as the first step for further crop improvement. Hypocotyls segment were tested on different media; best results for direct shoot organogenesis was achieved on medium fortified with 0.45 mg/l Thidiazuron + 3.0 mg/l benzyl adenine and 0.1 mg/l Naphthalene acetic acid (88.3%), with an average shoot number of 10.5 ± 1.23. For indirect shoot organogenesis, (and after an initial incubation of 10 days on 2, 4-dichlorophenoxy acetic acid fortified medium), the best results were obtained on medium supplemented with 0.15 mg/l Thidiazuron + 1.0 mg/l benzyl adenine and 0.5 mg/l naphthalene acetic acid (73.75%), with an average shoots of 7.38 ± 0.17. Achieving a reliable regeneration system is a step forward in production of genetically enhanced dehydration-stress tolerance varieties able to grow in new reclaimed area in Egypt.