Effect of Pesticide (Chlorpyrifos) on Soil Microbial Flora and Pesticide Degradation by Strains Isolated from Contaminated Soil

摘要

In the present study, the effect of pesticide (Chlorpyrifos) on rhizospheric soil and non–rhizospheric soil of two plants marigold and Canna has been investigated. Further, microorganisms have been isolated from Rhizospheric and Non–Rhizospheric soil, characterized and their pesticide degradation ability was investigated. Most of bio process materials have been taken and analyzed for microbial composition. The efficiency of microbial consortium obtained from each of this bio process material for chlorpyrifos degradation has been studied. The result indicates that presence of glucose supports more biomass, which in turn brings about higher degradation and dissipation of pesticide. Maximum 84.5% dissipation was observed through bacterial isolate in presence of glucose as compared to 73.3% dissipation in absence of glucose. In case of fungal isolate 76% dissipation occurred in presence of glucose and only 58% was dissipated in absence of glucose. Both the isolates showed resistance to chlorpyrifos at 10 ppm concentration and also brought about significant dissipation of this pesticide. Therefore, these isolated could be potential candidates for microbe mediated bioremediation of chlorpyrifos contaminated soils.