Biomass charcoal powder (BCP) was used as a carrier matrix for immobilization of chicken egg white lysozyme. BCP was derived from plant biomass wastes such as dumped adzuki beans by pyrolysis without combustion under nitrogen atmosphere and grinding with a jet mill. The amount of lysozyme immobilized on BCP of adzuki beans by adsorption was 11 μmol/g (0.16 g/g) at pH 7.0. The optimum pH values for free and immobilized lysozyme activities were 6.8 and 7.2, respectively. The optimum temperature for both free and immobilized lysozyme activities was 25℃. The half-life of immobilized lysozyme exhibited 1.8-fold compared to that of free lysozyme at 5℃. Moreover, the half life of immobilized lysozyme was 7 times greater than that of lysozyme at 90℃.
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机译：生物质木炭粉（BCP）用作固定化鸡蛋白溶菌酶的载体基质。 BCP源自植物生物质废物（例如倾倒的小豆），无需氮气氛下的热解即可进行热解，并用喷射磨进行研磨。在pH 7.0下，通过吸附固定在小豆BCP上的溶菌酶量为11μmol/ g（0.16 g / g）。游离和固定的溶菌酶活性的最佳pH值分别为6.8和7.2。游离和固定溶菌酶活性的最佳温度均为25℃。固定的溶菌酶在5℃下的半衰期是游离溶菌酶的1.8倍。而且，固定化的溶菌酶在90℃的半衰期是溶菌酶的7倍。
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