Anti-diabetic effect of metformin combined with peanut (Arachis hypogaea L.) on streptozotocin induced diabetic rats

摘要

Background: Diabetes mellitus (DM) is a common metabolic disorder. Metformin is the initial drug of choice for treatment of type 2 DM. In many cases, metformin mono-therapy cannot effectively achieve the targeted glycemic control. However, metformin along with peanut (Arachis hypogaea L.) may reduce blood glucose level more effectively.Objective: To evaluate the anti-diabetic effect of metformin in combination with peanut on streptozotocin induced diabetic rats.Methods: This experimental study was conducted in the Department of Physiology, Sir Salimullah Medical College, in 2016. Forty (40) Wistar Albino male rats, 90-120 days old, weighing 225-240 g (initial body weight) were taken and divided into four groups containing 10 rats in each group, i.e. Non-diabetic group (ND), Streptozotocin induced diabetic group (STZ), Diabetic group treated with metformin (STZ-M) and Diabetic group treated with metformin and peanut (STZ-MP). Diabetic model was developed by giving single intraperitoneal injection of streptozotocin (50mg/kg body weight) to STZ, STZ-M and STZ-MP groups on day-1. In addition, STZ-M group received metformin (500mg/kg body weight) orally and STZ-MP group received both metformin and peanut extract orally (both 500mg/kg body weight) once daily in the morning for 21 days (day-4 to day-24). After measuring the final body weight, rats were sacrificed on day-25. To observe glycemic status, Fasting Blood Glucose (FBG), serum insulin levels were estimated and HOMA-IR was calculated. Statistical analyses were done by one way ANOVA and Bonferroni test as applicable.Results: Mean FBG level and HOMA-IR were significantly (p 0.001) higher and serum insulin level and final body weight were significantly (p 0.001) lower in STZ group when compared to those of ND group. Whereas, FBG level and HOMA-IR were significantly (p 0.001) lower and insulin level and final body weight were significantly higher (p 0.001) in both STZ-M and STZ-MP groups in comparison to those of STZ group but more profound effects were found in STZ-MP group than those of STZ-M group.Conclusion: The present study revealed that, metformin combined with peanut was more effective to control glycemic status in diabetic rats than metformin alone.