Effect of Rooting Media With or Without Activated Charcoal, Auxin Type andArginine Concentrations on the Rooting Performance of Bitter Almond Micro-Cuttings

摘要

This study was carried out in plant tissue culture laboratory, Agriculture development system project (ADS) at Giza, Egypt during the period of 1999 – 2003. This study aimed to micropropagate – hard to root – bitter almond rootstock using tissue culture technique through studying the effect of different rooting media, activated charcool, auxin types and arginine on the rooting performance. From the obtained results the following conclusions were derived: WP medium proved to be suitable rooting medium for rooting bitter almond shoots followed by B&N and MS respectively as it gave the highest rooting percentage , number of roots/rooted shoot while B&N and WP medium gave the longest root length. Adding activated charcoal to the rooting medium cause a sharp decrease in the best rooting percentage. NAA was the most effective auxin in inducing the best rooting percentage (40.74), greatest number of roots/rooted shoot and the longest root of bitter almond shoots followed by IBA, while IAA took the other way around. Adding arginine at 100.0 mg /L to the rooting medium (WPM) enhanced the rooting percentage and number of roots/explants.