Characterization and selection of halophilic microorganisms isolated from Mexican soils

摘要

Halophilic microorganisms thrive in conditions of high salt concentration and osmotic stress. Due to these reasons, halophilic microorganisms with ability to fix nitrogen or mobilize phosphate represent a potential as bio-fertilizer to increase crop growth and yield in saline soils. In this study, 35 different halophilic bacteria were isolated from saline soils of Coahuila State, Mexico. These bacterial isolates were characterized, and evaluated for their halophilic potential by growth kinetics, hydrolysis halos formation and in vitro sodium capture. Based on the results, seven potential strains for saline conditions were selected and were identified by 16S rDNA analysis. Phylogenetic relationship of the selected strains as well as with other halophilic microorganisms was determined. Six of the identified strains were Halobacillus trueperi , Bacillus licheniformis , Bacillus pumilus , Staphylococcus succinus , Bacillus atrophaeus and Bacillus subtilis . Other two strains identified were Halobacillus sp. and Oceanobacillus sp., moderately halophilic organisms.