Polymorphism on mtDNA D-loop and ND5 Regions Among Turkish Native Cattle Breeds Assessed by PCR-RFLP

摘要

Ozdemir, M., Dogru, U. and Ciftci, M. 2009. Polymorphism on mtDNA D-loop and ND5 regions among Turkish native cattle breeds assessed by PCR-RFLP. J. Appl Anim. Res., 36: 37?￠????40. To determine the specific polymorphic sites of Turkish native cattle breeds (Turkish Grey, East Anatolian Red, South Anatolian Red and Anatolian Black) and genetic variations in mtDNA entire D-loop region with high mutation rate and 1100 bp of constant ND5 region were analyzed. In 910 bp of D-loop region, Alu I, Hae III, Hpa II, Ava II, Dra I and Apa I enzymes from ten restriction endonucleases, which have 17 restriction sites, showed polymorphic sites and 19 haplotypes were found. Alu I, Hae III, Bgl I???- and Ssp I enzymes from ten restriction endonucleases, which have 25 restriction sites representing polymorphic sites, and 6 haplotypes were found in the ND5 region. Results of the cluster analysis carried out on the D-loop and ND5 region were similar to each other. East Anatolian Red, South Anatolian Red and Anatolian Black formed the common group distant from Turkish Grey.