Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli

摘要

Duringconstructionofgenomiclibrariesfromvariousbacteria,weexperiencedconsiderablydifferenttransformationefficienciesamongthestrainsofelectrocompetentEscherichiacolicellsandinvestigatedtheeffectoftypestrainonefficiencyoftransformation.Amongthefivestrainstested,EscherichiacoliDH10BandTOP10werefoundtobethemostsuitablestrainsforconstructionofgenomiclibrariesinourlaboratory.ThisstudyhighlightedthefactthatalthoughthesecommerciallyavailableEscherichiacolistrainsarewellcharacterized,theytendtodifferentiateandloosetheirtransformationabilities.Thismaybeduetothefactthatthenumberofpassagestheyhavegonethroughaffecttheirmembranestructuresandpermeability.Therefore,everylaboratoryshouldtesttheirstrainsforcompetencybeforetheyusethemintheircloningstudies.