High-Throughput Analysis of Lidocaine in Pharmaceutical Formulation by Capillary Zone Electrophoresis Using Multiple Injections in a Single Run

摘要

This paper reports the development of a subminute separation method by capillary zone electrophoresis in an uncoated capillary using multiple injection procedure for the determination of lidocaine in samples of pharmaceutical formulations. The separation was performed in less than a minute leading to doing four injections in a single run. The cathodic electroosmotic flow contributed to reducing the analyses time. The background electrolyte was composed of 20?mmol?L?1 2-amino-2-(hydroxymethyl)-1,3-propanediol and 40?mmol?L?1 2-(N-morpholino)ethanesulfonic acid at pH 6.1. The internal standard used was benzylamine. Separations were performed in a fused uncoated silica capillary (32?cm total length, 23.5?cm effective length, and 50??m internal diameter) with direct UV detection at 200?nm. Samples and standards were injected hydrodynamically using 40?mbar/3?s interspersed with spacer electrolyte using 40?mbar/7?s. The electrophoretic system was operated under constant voltage of 30?kV with positive polarity on the injection side. The evaluation of some analytical parameters of the method showed good linearity ( r 2 > 0.999 ) , a limit of detection 0.92?mg?L?1, intermediate precision better than 3.2% (peak area), and recovery in the range of 92–102%.