cDNA Cloning, Sequence Identification and Tissue Expression Profile of a Novel Gene FAR1 From Banna Mini-Pig Inbred Line (BMI)

摘要

The complete CDS sequence of Banna Mini-pig Inbred line (BMI) gene FAR1 was amplified using the Reverse Transcriptase-polymerase Chain Reaction (RT-PCR) based on the conserved sequence information of the cattle or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and assigned to Accession No.: JF944893. Sequence analysis revealed that the BMI FAR1 encodes a protein of 515 amino acids that has high homology with the fatty acyl-CoA reductase 1 proteins of seven species-cattle 98%, horse 98%, mouse 98%, orangutan 97%, human 97%, monkey 97% and rat 92%. The phylogenetic tree analysis revealed BMI FAR1 has a closer genetic relationship with the bovine, human, orangutan and monkey FAR1 than with those of horse, mouse and rat. Analysis by RT-PCR showed that BMI FAR1 gene was over-expressed in muscle, lung, ovary, skin, large intestine, spleen, small intestine and nerve fiber and almost not expressed in other 10 tissues. Several microRNA target sites were predicted in the CDS of BMI FAR1 mRNA for further studying this gene in the future. The 3D structure of the FAR1 by homology modeling was similar to that of pseudomonas 3-alpha-hydroxysteroid dehydrogenase (2 dkn Chain: A). The experiment will establish a foundation for further insight into this swine gene.