Fibrous bone tissue engineering scaffolds prepared by wet spinning of PLGA

摘要

Having a self-healing capacity, bone is very well known to regenerate itself without leaving a scar. However, critical size defectsdue to trauma, tumor, disease, or infection involve bone graft surgeries in which complication rate is relatively at high levels. Bone tissueengineering appears as an alternative for grafting. Fibrous scaffolds are useful in tissue engineering applications since they have a highsurface-to-volume ratio, and adjustable, highly interconnected porosity to enhance cell adhesion, survival, migration, and proliferation.They can be produced in a wide variety of fiber sizes and organizations. Wet spinning is a convenient way to produce fibrous scaffoldswith consistent fiber size and good mechanical properties. In this study, a fibrous bone tissue engineering scaffold was produced usingpoly(lactic-co-glycolic acid) (PLGA). Different concentrations (20%, 25%, and 30%) of PLGA (PLAPGA 7525) (Mw = 66,000?107,000)were wet spun using coagulation baths composed of different ratios (7525, 6040, 5050) of isopropanol and distilled water. Scanningelectron microscopy (SEM) and in vitro degradation studies were performed to characterize the fibrous PLGA scaffolds. Mesenchymalstem cells were isolated from rat bone marrow, characterized by flow cytometry and seeded onto scaffolds to determine the mostappropriate fibrous structure for cell proliferation. According to the results of SEM, degradation studies and cell proliferation assay, 20%PLGA wet spun in 6040 coagulation bath was selected as the most successful condition for the preparation of wet-spun scaffolds. Wetspinning of different concentrations of PLGA (20%, 25%, 30%) dissolved in dichloromethane using different isopropanoldistilled waterratios of coagulation baths (7525, 6040, 5050) were shown in this study.