Comparison Crude Antigens of Fasciolosis hepatica and Candida albicans byMolecular Tests: A way to Drug

摘要

Early diagnosis is very important for fasciolosis and Candidiasis in prevention and control. Two productive species, namely F. hepatica and Candida Albicans of the disease in terms of together morphological similarities in some of the features of a great but the differences. In this study, the presence or absence of similarity between genes raw of Antique excretory - secretory and somatic two species of the parasite and Fungi to Elisa test was evaluated. Crude antigens of both species (including excretory - secretory and SCC) developed and to use in the cold ºc -20 were kept. In the preparing the antiserum, antigens with the increasing the dose 0 / 5-2 / 5 in the five times with intervals of seven days, two rabbits were injected. Ten days after the last injection, blood and serum samples after separation, in cold 20 ° C were kept. Reaction of Crude antigen the antiserum any species with the self antiserum (homologous) and as well as antiserum other species (heterologous) evaluated by ELISA and ELISA values were recorded. When the reaction with the antiserum-antigen ELISA values of any other species in comparison with control samples showed that with the antiserum antigen any kind other species show that a strong reaction. Meaning that of antigenic cross-reactivity in ELISA two species is similar and there. However, antigens of F. hepatica and Candida Albicans with the antiserum a stronger reaction than the antiserum were found Despite the similarity between many of antigenic material of two species, some of these substances are different. F. hepatica and Candida Albicans antigenic differences between the materials is not enough to prevent cross-reaction between the two species and more research is geared in ELISA for the detection, isolation and purification of antigenic materials of any recommended.